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anti m13 antibody  (Sino Biological)
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Sino Biological anti m13 antibody
Identification and characterization of KRAS G12V/HLA-A*11:01 specific scFv. ( A ) Screening of specific phage clones to the KRAS G12V/HLA-A*11:01 complex by ELISA. KRAS G12V/HLA-A*11:01, KRAS WT/HLA-A*11:01 or empty HLA-A*11:01 monomers were bound to the plate and then incubated with supernatant of the phage clones, followed by detection with an <t>anti-M13</t> antibody. Optical density at 450 nm was measured on a spectrophotometric plate reader. Data represent mean±SD of three technical replicates. ( B ) Binding of the scFvs (scFv1 and scFv2 from phage clones 19 and 27, respectively) to the KRAS G12V/HLA-A*11:01 complex was assessed by ELISA. KRAS G12V/HLA-A*11:01 or KRAS WT/HLA-A*11:01 monomers were bound to the plate and then incubated with the purified scFvs at 10 nM, followed by detection with an anti-His antibody. Optical density at 450 nm was measured on a spectrophotometric plate reader. Data represent mean±SD of three technical replicates. ( C-D ) Flow cytometry analysis of scFv binding specificity to KRAS G12V/HLA-A*11:01 complexes. ( C ) C1R-A11 cells pulsed with KRAS G12V, KRAS WT or other KRAS G12 variant peptides (G12A, G12C, G12D, G12R and G12S) were incubated with scFv, followed by detection with an anti-FLAG PE antibody. Histograms show PE fluorescence intensity. ( D ) Cancer cell lines with various combinations of KRAS mutation status and HLA-A types were similarly analyzed for scFv binding. An unrelated APC neoantigen-specific scFv was used as isotype control. Histograms show PE fluorescence intensity. Data represent mean±SD of three technical replicates. APC, adenomatous polyposis coli; HLA, human leukocyte antigen; PE, phycoerythrin; scFv, single-chain variable fragment; WT, wild-type.
Anti M13 Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+m13+mab/M13+Bacteriophage+Antibody%2C+Mouse+Mab/pmc13691759-54-17-19
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anti m13 antibody - by Bioz Stars, 2026-11
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hrp conjugated anti m13 monoclonal antibody  (Sino Biological)
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Identification and characterization of KRAS G12V/HLA-A*11:01 specific scFv. ( A ) Screening of specific phage clones to the KRAS G12V/HLA-A*11:01 complex by ELISA. KRAS G12V/HLA-A*11:01, KRAS WT/HLA-A*11:01 or empty HLA-A*11:01 monomers were bound to the plate and then incubated with supernatant of the phage clones, followed by detection with an <t>anti-M13</t> antibody. Optical density at 450 nm was measured on a spectrophotometric plate reader. Data represent mean±SD of three technical replicates. ( B ) Binding of the scFvs (scFv1 and scFv2 from phage clones 19 and 27, respectively) to the KRAS G12V/HLA-A*11:01 complex was assessed by ELISA. KRAS G12V/HLA-A*11:01 or KRAS WT/HLA-A*11:01 monomers were bound to the plate and then incubated with the purified scFvs at 10 nM, followed by detection with an anti-His antibody. Optical density at 450 nm was measured on a spectrophotometric plate reader. Data represent mean±SD of three technical replicates. ( C-D ) Flow cytometry analysis of scFv binding specificity to KRAS G12V/HLA-A*11:01 complexes. ( C ) C1R-A11 cells pulsed with KRAS G12V, KRAS WT or other KRAS G12 variant peptides (G12A, G12C, G12D, G12R and G12S) were incubated with scFv, followed by detection with an anti-FLAG PE antibody. Histograms show PE fluorescence intensity. ( D ) Cancer cell lines with various combinations of KRAS mutation status and HLA-A types were similarly analyzed for scFv binding. An unrelated APC neoantigen-specific scFv was used as isotype control. Histograms show PE fluorescence intensity. Data represent mean±SD of three technical replicates. APC, adenomatous polyposis coli; HLA, human leukocyte antigen; PE, phycoerythrin; scFv, single-chain variable fragment; WT, wild-type.
Hrp Conjugated Anti M13 Monoclonal Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+m13+mab/M13+Bacteriophage+Antibody+(HRP)%2C+Mouse+Mab/bio_rxiv__64898__2026__10__08__757526-448-6-10
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hrp conjugated anti m13 monoclonal antibody - by Bioz Stars, 2026-11
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hrp conjugated mouse anti m13 antibody  (Sino Biological)
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Sino Biological hrp conjugated mouse anti m13 antibody
Identification and characterization of KRAS G12V/HLA-A*11:01 specific scFv. ( A ) Screening of specific phage clones to the KRAS G12V/HLA-A*11:01 complex by ELISA. KRAS G12V/HLA-A*11:01, KRAS WT/HLA-A*11:01 or empty HLA-A*11:01 monomers were bound to the plate and then incubated with supernatant of the phage clones, followed by detection with an <t>anti-M13</t> antibody. Optical density at 450 nm was measured on a spectrophotometric plate reader. Data represent mean±SD of three technical replicates. ( B ) Binding of the scFvs (scFv1 and scFv2 from phage clones 19 and 27, respectively) to the KRAS G12V/HLA-A*11:01 complex was assessed by ELISA. KRAS G12V/HLA-A*11:01 or KRAS WT/HLA-A*11:01 monomers were bound to the plate and then incubated with the purified scFvs at 10 nM, followed by detection with an anti-His antibody. Optical density at 450 nm was measured on a spectrophotometric plate reader. Data represent mean±SD of three technical replicates. ( C-D ) Flow cytometry analysis of scFv binding specificity to KRAS G12V/HLA-A*11:01 complexes. ( C ) C1R-A11 cells pulsed with KRAS G12V, KRAS WT or other KRAS G12 variant peptides (G12A, G12C, G12D, G12R and G12S) were incubated with scFv, followed by detection with an anti-FLAG PE antibody. Histograms show PE fluorescence intensity. ( D ) Cancer cell lines with various combinations of KRAS mutation status and HLA-A types were similarly analyzed for scFv binding. An unrelated APC neoantigen-specific scFv was used as isotype control. Histograms show PE fluorescence intensity. Data represent mean±SD of three technical replicates. APC, adenomatous polyposis coli; HLA, human leukocyte antigen; PE, phycoerythrin; scFv, single-chain variable fragment; WT, wild-type.
Hrp Conjugated Mouse Anti M13 Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+m13+mab/M13+Bacteriophage+Antibody+(HRP)%2C+Mouse+Mab/10__3390_slash_ijms27209074-277-20-24
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hrp conjugated mouse anti m13 antibody - by Bioz Stars, 2026-11
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horseradish peroxidase hrp conjugated anti m13 pviii antibody  (Sino Biological)
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Sino Biological horseradish peroxidase hrp conjugated anti m13 pviii antibody
Identification and characterization of KRAS G12V/HLA-A*11:01 specific scFv. ( A ) Screening of specific phage clones to the KRAS G12V/HLA-A*11:01 complex by ELISA. KRAS G12V/HLA-A*11:01, KRAS WT/HLA-A*11:01 or empty HLA-A*11:01 monomers were bound to the plate and then incubated with supernatant of the phage clones, followed by detection with an <t>anti-M13</t> antibody. Optical density at 450 nm was measured on a spectrophotometric plate reader. Data represent mean±SD of three technical replicates. ( B ) Binding of the scFvs (scFv1 and scFv2 from phage clones 19 and 27, respectively) to the KRAS G12V/HLA-A*11:01 complex was assessed by ELISA. KRAS G12V/HLA-A*11:01 or KRAS WT/HLA-A*11:01 monomers were bound to the plate and then incubated with the purified scFvs at 10 nM, followed by detection with an anti-His antibody. Optical density at 450 nm was measured on a spectrophotometric plate reader. Data represent mean±SD of three technical replicates. ( C-D ) Flow cytometry analysis of scFv binding specificity to KRAS G12V/HLA-A*11:01 complexes. ( C ) C1R-A11 cells pulsed with KRAS G12V, KRAS WT or other KRAS G12 variant peptides (G12A, G12C, G12D, G12R and G12S) were incubated with scFv, followed by detection with an anti-FLAG PE antibody. Histograms show PE fluorescence intensity. ( D ) Cancer cell lines with various combinations of KRAS mutation status and HLA-A types were similarly analyzed for scFv binding. An unrelated APC neoantigen-specific scFv was used as isotype control. Histograms show PE fluorescence intensity. Data represent mean±SD of three technical replicates. APC, adenomatous polyposis coli; HLA, human leukocyte antigen; PE, phycoerythrin; scFv, single-chain variable fragment; WT, wild-type.
Horseradish Peroxidase Hrp Conjugated Anti M13 Pviii Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+m13+mab/M13+Bacteriophage+Antibody+(HRP)%2C+Mouse+Mab/10__3390_slash_biom16101379-90-9-15
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horseradish peroxidase hrp conjugated anti m13 pviii antibody - by Bioz Stars, 2026-11
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anti m13 hrp antibody  (Sino Biological)
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Sino Biological anti m13 hrp antibody
Identification and characterization of KRAS G12V/HLA-A*11:01 specific scFv. ( A ) Screening of specific phage clones to the KRAS G12V/HLA-A*11:01 complex by ELISA. KRAS G12V/HLA-A*11:01, KRAS WT/HLA-A*11:01 or empty HLA-A*11:01 monomers were bound to the plate and then incubated with supernatant of the phage clones, followed by detection with an <t>anti-M13</t> antibody. Optical density at 450 nm was measured on a spectrophotometric plate reader. Data represent mean±SD of three technical replicates. ( B ) Binding of the scFvs (scFv1 and scFv2 from phage clones 19 and 27, respectively) to the KRAS G12V/HLA-A*11:01 complex was assessed by ELISA. KRAS G12V/HLA-A*11:01 or KRAS WT/HLA-A*11:01 monomers were bound to the plate and then incubated with the purified scFvs at 10 nM, followed by detection with an anti-His antibody. Optical density at 450 nm was measured on a spectrophotometric plate reader. Data represent mean±SD of three technical replicates. ( C-D ) Flow cytometry analysis of scFv binding specificity to KRAS G12V/HLA-A*11:01 complexes. ( C ) C1R-A11 cells pulsed with KRAS G12V, KRAS WT or other KRAS G12 variant peptides (G12A, G12C, G12D, G12R and G12S) were incubated with scFv, followed by detection with an anti-FLAG PE antibody. Histograms show PE fluorescence intensity. ( D ) Cancer cell lines with various combinations of KRAS mutation status and HLA-A types were similarly analyzed for scFv binding. An unrelated APC neoantigen-specific scFv was used as isotype control. Histograms show PE fluorescence intensity. Data represent mean±SD of three technical replicates. APC, adenomatous polyposis coli; HLA, human leukocyte antigen; PE, phycoerythrin; scFv, single-chain variable fragment; WT, wild-type.
Anti M13 Hrp Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+m13+mab/M13+Bacteriophage+Antibody+(HRP)%2C+Mouse+Mab/us12606628-235-11-15
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tmb substrate  (Sino Biological)
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Identification and characterization of KRAS G12V/HLA-A*11:01 specific scFv. ( A ) Screening of specific phage clones to the KRAS G12V/HLA-A*11:01 complex by ELISA. KRAS G12V/HLA-A*11:01, KRAS WT/HLA-A*11:01 or empty HLA-A*11:01 monomers were bound to the plate and then incubated with supernatant of the phage clones, followed by detection with an <t>anti-M13</t> antibody. Optical density at 450 nm was measured on a spectrophotometric plate reader. Data represent mean±SD of three technical replicates. ( B ) Binding of the scFvs (scFv1 and scFv2 from phage clones 19 and 27, respectively) to the KRAS G12V/HLA-A*11:01 complex was assessed by ELISA. KRAS G12V/HLA-A*11:01 or KRAS WT/HLA-A*11:01 monomers were bound to the plate and then incubated with the purified scFvs at 10 nM, followed by detection with an anti-His antibody. Optical density at 450 nm was measured on a spectrophotometric plate reader. Data represent mean±SD of three technical replicates. ( C-D ) Flow cytometry analysis of scFv binding specificity to KRAS G12V/HLA-A*11:01 complexes. ( C ) C1R-A11 cells pulsed with KRAS G12V, KRAS WT or other KRAS G12 variant peptides (G12A, G12C, G12D, G12R and G12S) were incubated with scFv, followed by detection with an anti-FLAG PE antibody. Histograms show PE fluorescence intensity. ( D ) Cancer cell lines with various combinations of KRAS mutation status and HLA-A types were similarly analyzed for scFv binding. An unrelated APC neoantigen-specific scFv was used as isotype control. Histograms show PE fluorescence intensity. Data represent mean±SD of three technical replicates. APC, adenomatous polyposis coli; HLA, human leukocyte antigen; PE, phycoerythrin; scFv, single-chain variable fragment; WT, wild-type.
Tmb Substrate, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti m13 mouse monoclonal antibodies  (Sino Biological)
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Sino Biological anti m13 mouse monoclonal antibodies
Identification and characterization of KRAS G12V/HLA-A*11:01 specific scFv. ( A ) Screening of specific phage clones to the KRAS G12V/HLA-A*11:01 complex by ELISA. KRAS G12V/HLA-A*11:01, KRAS WT/HLA-A*11:01 or empty HLA-A*11:01 monomers were bound to the plate and then incubated with supernatant of the phage clones, followed by detection with an <t>anti-M13</t> antibody. Optical density at 450 nm was measured on a spectrophotometric plate reader. Data represent mean±SD of three technical replicates. ( B ) Binding of the scFvs (scFv1 and scFv2 from phage clones 19 and 27, respectively) to the KRAS G12V/HLA-A*11:01 complex was assessed by ELISA. KRAS G12V/HLA-A*11:01 or KRAS WT/HLA-A*11:01 monomers were bound to the plate and then incubated with the purified scFvs at 10 nM, followed by detection with an anti-His antibody. Optical density at 450 nm was measured on a spectrophotometric plate reader. Data represent mean±SD of three technical replicates. ( C-D ) Flow cytometry analysis of scFv binding specificity to KRAS G12V/HLA-A*11:01 complexes. ( C ) C1R-A11 cells pulsed with KRAS G12V, KRAS WT or other KRAS G12 variant peptides (G12A, G12C, G12D, G12R and G12S) were incubated with scFv, followed by detection with an anti-FLAG PE antibody. Histograms show PE fluorescence intensity. ( D ) Cancer cell lines with various combinations of KRAS mutation status and HLA-A types were similarly analyzed for scFv binding. An unrelated APC neoantigen-specific scFv was used as isotype control. Histograms show PE fluorescence intensity. Data represent mean±SD of three technical replicates. APC, adenomatous polyposis coli; HLA, human leukocyte antigen; PE, phycoerythrin; scFv, single-chain variable fragment; WT, wild-type.
Anti M13 Mouse Monoclonal Antibodies, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+m13+mab/M13+Bacteriophage+Antibody%2C+Mouse+Mab/us12595302-626-54-58
Average 96 stars, based on 1 article reviews
anti m13 mouse monoclonal antibodies - by Bioz Stars, 2026-11
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anti m13 mouse monoclonal antibody  (Sino Biological)
96
Sino Biological anti m13 mouse monoclonal antibody
Identification and characterization of KRAS G12V/HLA-A*11:01 specific scFv. ( A ) Screening of specific phage clones to the KRAS G12V/HLA-A*11:01 complex by ELISA. KRAS G12V/HLA-A*11:01, KRAS WT/HLA-A*11:01 or empty HLA-A*11:01 monomers were bound to the plate and then incubated with supernatant of the phage clones, followed by detection with an <t>anti-M13</t> antibody. Optical density at 450 nm was measured on a spectrophotometric plate reader. Data represent mean±SD of three technical replicates. ( B ) Binding of the scFvs (scFv1 and scFv2 from phage clones 19 and 27, respectively) to the KRAS G12V/HLA-A*11:01 complex was assessed by ELISA. KRAS G12V/HLA-A*11:01 or KRAS WT/HLA-A*11:01 monomers were bound to the plate and then incubated with the purified scFvs at 10 nM, followed by detection with an anti-His antibody. Optical density at 450 nm was measured on a spectrophotometric plate reader. Data represent mean±SD of three technical replicates. ( C-D ) Flow cytometry analysis of scFv binding specificity to KRAS G12V/HLA-A*11:01 complexes. ( C ) C1R-A11 cells pulsed with KRAS G12V, KRAS WT or other KRAS G12 variant peptides (G12A, G12C, G12D, G12R and G12S) were incubated with scFv, followed by detection with an anti-FLAG PE antibody. Histograms show PE fluorescence intensity. ( D ) Cancer cell lines with various combinations of KRAS mutation status and HLA-A types were similarly analyzed for scFv binding. An unrelated APC neoantigen-specific scFv was used as isotype control. Histograms show PE fluorescence intensity. Data represent mean±SD of three technical replicates. APC, adenomatous polyposis coli; HLA, human leukocyte antigen; PE, phycoerythrin; scFv, single-chain variable fragment; WT, wild-type.
Anti M13 Mouse Monoclonal Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+m13+mab/M13+Bacteriophage+Antibody%2C+Mouse+Mab/pm41825926-39-44-51
Average 96 stars, based on 1 article reviews
anti m13 mouse monoclonal antibody - by Bioz Stars, 2026-11
96/100 stars
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Identification and characterization of KRAS G12V/HLA-A*11:01 specific scFv. ( A ) Screening of specific phage clones to the KRAS G12V/HLA-A*11:01 complex by ELISA. KRAS G12V/HLA-A*11:01, KRAS WT/HLA-A*11:01 or empty HLA-A*11:01 monomers were bound to the plate and then incubated with supernatant of the phage clones, followed by detection with an anti-M13 antibody. Optical density at 450 nm was measured on a spectrophotometric plate reader. Data represent mean±SD of three technical replicates. ( B ) Binding of the scFvs (scFv1 and scFv2 from phage clones 19 and 27, respectively) to the KRAS G12V/HLA-A*11:01 complex was assessed by ELISA. KRAS G12V/HLA-A*11:01 or KRAS WT/HLA-A*11:01 monomers were bound to the plate and then incubated with the purified scFvs at 10 nM, followed by detection with an anti-His antibody. Optical density at 450 nm was measured on a spectrophotometric plate reader. Data represent mean±SD of three technical replicates. ( C-D ) Flow cytometry analysis of scFv binding specificity to KRAS G12V/HLA-A*11:01 complexes. ( C ) C1R-A11 cells pulsed with KRAS G12V, KRAS WT or other KRAS G12 variant peptides (G12A, G12C, G12D, G12R and G12S) were incubated with scFv, followed by detection with an anti-FLAG PE antibody. Histograms show PE fluorescence intensity. ( D ) Cancer cell lines with various combinations of KRAS mutation status and HLA-A types were similarly analyzed for scFv binding. An unrelated APC neoantigen-specific scFv was used as isotype control. Histograms show PE fluorescence intensity. Data represent mean±SD of three technical replicates. APC, adenomatous polyposis coli; HLA, human leukocyte antigen; PE, phycoerythrin; scFv, single-chain variable fragment; WT, wild-type.

Journal: Journal for Immunotherapy of Cancer

Article Title: Engineering T cell-engaging bispecific antibodies targeting KRAS G12V neoantigen across HLA-A*11:01 and HLA-A*03:01

doi: 10.1136/jitc-2026-016411

Figure Lengend Snippet: Identification and characterization of KRAS G12V/HLA-A*11:01 specific scFv. ( A ) Screening of specific phage clones to the KRAS G12V/HLA-A*11:01 complex by ELISA. KRAS G12V/HLA-A*11:01, KRAS WT/HLA-A*11:01 or empty HLA-A*11:01 monomers were bound to the plate and then incubated with supernatant of the phage clones, followed by detection with an anti-M13 antibody. Optical density at 450 nm was measured on a spectrophotometric plate reader. Data represent mean±SD of three technical replicates. ( B ) Binding of the scFvs (scFv1 and scFv2 from phage clones 19 and 27, respectively) to the KRAS G12V/HLA-A*11:01 complex was assessed by ELISA. KRAS G12V/HLA-A*11:01 or KRAS WT/HLA-A*11:01 monomers were bound to the plate and then incubated with the purified scFvs at 10 nM, followed by detection with an anti-His antibody. Optical density at 450 nm was measured on a spectrophotometric plate reader. Data represent mean±SD of three technical replicates. ( C-D ) Flow cytometry analysis of scFv binding specificity to KRAS G12V/HLA-A*11:01 complexes. ( C ) C1R-A11 cells pulsed with KRAS G12V, KRAS WT or other KRAS G12 variant peptides (G12A, G12C, G12D, G12R and G12S) were incubated with scFv, followed by detection with an anti-FLAG PE antibody. Histograms show PE fluorescence intensity. ( D ) Cancer cell lines with various combinations of KRAS mutation status and HLA-A types were similarly analyzed for scFv binding. An unrelated APC neoantigen-specific scFv was used as isotype control. Histograms show PE fluorescence intensity. Data represent mean±SD of three technical replicates. APC, adenomatous polyposis coli; HLA, human leukocyte antigen; PE, phycoerythrin; scFv, single-chain variable fragment; WT, wild-type.

Article Snippet: After six washes with TBST (Tris-buffered saline (TBS) with 0.05% Tween-20), bound phages were detected using an anti-M13 antibody (Sino Biological, Beijing, China).

Techniques: Screening, Clone Assay, Enzyme-linked Immunosorbent Assay, Incubation, Binding Assay, Purification, Flow Cytometry, Analysis, Variant Assay, Fluorescence, Mutagenesis, Control